Detection of mutations conferring extended-spectrum activity on SHV β-lactamases using polymerase chain reaction single strand conformational polymorphism (PCR-SSCP)

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F-SSCP: fluorescence-based polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis.

A fluorescence-based method for polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis, F-SSCP, was developed in which the target sequence is amplified by the PCR using fluorescent primers. The amplified products are then heat-denatured and applied to a water-jacket controlled gel in an automated DNA sequencer. The separated strands are detected as laser-excited f...

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Genetic Polymorphism at the Growth Hormone Locus in Iranian Talli Goats by Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP)

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Analysis of p53 gene mutations in keloids using polymerase chain reaction-based single-strand conformational polymorphism and DNA sequencing.

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ژورنال

عنوان ژورنال: Journal of Antimicrobial Chemotherapy

سال: 1996

ISSN: 0305-7453,1460-2091

DOI: 10.1093/jac/37.4.797